|｢ｺ An alternative method for rat fixation when giving subcutaneous, intramuscular and intraperitoneal injections ("Camilla｢･s method")|
Scandinavian Journal of Laboratory Animal Science 1999, 26(3), 156-159.
The usual way of fixing rats is by taking them in the scruff of the neck or around the front quarters, as described in several handbooks (Svendsen & Hau 1994, Van Zutphen et al. 1993). One of our animal technicians has developed another method, which is considered less stressful for the people executing them, thereby leading to more secure applications. The method may also be less stressful for the rats, as the head of the rat remains in the dark while performing the injections. This may be of advantage when premedication for the induction of anesthesia is given, because a more quiet induction will give a stronger effect, thereby reducing the amount of anesthetics needed. Also, when people are less stressed when performing the procedures, it is likely that rats will be less stressed. Furthermore, it is easy to perform the method when one does not have any assistance at hand.
For all methods described it is essential to prepare well, so that the injections can be performed quickly and smoothly, with the least distress for people and animals. The techniques that are described in the following, are written for a right-handed person, i.e. uses the right hand for injecting. For all three methods described, a 1 ml syringe is used with a 25 Gauge needle (0.5 mm external diameter) of 1.5 cm length. A relatively small and short needle is necessary when applying intramuscular and intraperitoneal injections.
Subcutaneous (sc) injection
The method for subcutanous injection is executed as follows.
The rat is put onto a cloth or a towel, so that there is a length of about 15 cm cranially of the head of the rat.
Intramuscular (im) injection
Intraperitoneal (ip) injection
In our experience, these 3 described methods function well in practice, especially with relatively inexperienced researchers. It calms people down and our subjective impression is that rats behave calmer as well.
It would need more research to prove whether rats indeed would be less stressed by using this alternative fixation method.
Svendsen P & J Hau (eds.): Handbook of Laboratory Animal Science, volume I, Selection and handling of animals in biomedical research. CRC Press, 1994.
Van Zutphen LFM, V Baumans & AC Beynen (eds.): Principles of Laboratory Animal Science. Elsevier, 1993.
Saturday, July 9, 2011
Macam macam cara injeksi rat (SM, IP, SC)
Artikel ini sangat bagus menjelaskan cara menginjeksi rat melalui 3 cara :im,ip,sc